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plasmid dna bluescript ii sk  (Qiagen)


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    Structured Review

    Qiagen plasmid dna bluescript ii sk
    (a) The panel shows the different mixtures of <t>DNA</t> (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound <t>plasmid</t> <t>DNA</t> to the peptide by gel densitometry analysis.
    Plasmid Dna Bluescript Ii Sk, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna bluescript ii sk/product/Qiagen
    Average 90 stars, based on 1 article reviews
    plasmid dna bluescript ii sk - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans"

    Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0069316

    (a) The panel shows the different mixtures of DNA (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound plasmid DNA to the peptide by gel densitometry analysis.
    Figure Legend Snippet: (a) The panel shows the different mixtures of DNA (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound plasmid DNA to the peptide by gel densitometry analysis.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    (a) Measurement of intrinsic tryptophan fluorescence of MMGP1 on addition of plasmid DNA (b) Quenching of SYTO9 fluorescence in the presence of varying concentrations of MMGP1.
    Figure Legend Snippet: (a) Measurement of intrinsic tryptophan fluorescence of MMGP1 on addition of plasmid DNA (b) Quenching of SYTO9 fluorescence in the presence of varying concentrations of MMGP1.

    Techniques Used: Fluorescence, Plasmid Preparation

    (a) Treatment of DNA–MMGP1 complexes with proteinase K resulted in detection of plasmid DNA band on the agarose gel. C-Control SK+ plasmid DNA; 1-DNA–MMGP1 complex formed at 0.576 µM concentration of peptide; 2-DNA–MMGP1 complex treated with proteinase K (b) DNase I protection assay. The plasmid DNA (100 ng) was preincubated with the varying concentrations peptides such as, C+-no peptide; 1-0.576 µM; 2-0.288 µM; 3- 0.144 μM; 4-0.072 µM; 5-0.036 µM, respectively followed by treatment with DNase I. The DNase treated plasmid DNA was used as negative control (C-).
    Figure Legend Snippet: (a) Treatment of DNA–MMGP1 complexes with proteinase K resulted in detection of plasmid DNA band on the agarose gel. C-Control SK+ plasmid DNA; 1-DNA–MMGP1 complex formed at 0.576 µM concentration of peptide; 2-DNA–MMGP1 complex treated with proteinase K (b) DNase I protection assay. The plasmid DNA (100 ng) was preincubated with the varying concentrations peptides such as, C+-no peptide; 1-0.576 µM; 2-0.288 µM; 3- 0.144 μM; 4-0.072 µM; 5-0.036 µM, respectively followed by treatment with DNase I. The DNase treated plasmid DNA was used as negative control (C-).

    Techniques Used: Plasmid Preparation, Agarose Gel Electrophoresis, Control, Concentration Assay, Negative Control



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    (a) The panel shows the different mixtures of <t>DNA</t> (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound <t>plasmid</t> <t>DNA</t> to the peptide by gel densitometry analysis.
    Plasmid Dna Bluescript Ii Sk, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna bluescript ii sk/product/Qiagen
    Average 90 stars, based on 1 article reviews
    plasmid dna bluescript ii sk - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) The panel shows the different mixtures of DNA (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound plasmid DNA to the peptide by gel densitometry analysis.

    Journal: PLoS ONE

    Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

    doi: 10.1371/journal.pone.0069316

    Figure Lengend Snippet: (a) The panel shows the different mixtures of DNA (100 ng) and MMGP1 (1- 0.036; 2- 0.072; 3- 0.144; 4- 0.288; 5-0.576 µM; C-without peptide) run on a 1% agarose gel. (b) Quantitative assessment of unbound plasmid DNA to the peptide by gel densitometry analysis.

    Article Snippet: The plasmid DNA Bluescript II SK (+) was purified using a QIAprep Spin Minprep kit (Qiagen, Germay) and used for subsequent analysis.

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation

    (a) Measurement of intrinsic tryptophan fluorescence of MMGP1 on addition of plasmid DNA (b) Quenching of SYTO9 fluorescence in the presence of varying concentrations of MMGP1.

    Journal: PLoS ONE

    Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

    doi: 10.1371/journal.pone.0069316

    Figure Lengend Snippet: (a) Measurement of intrinsic tryptophan fluorescence of MMGP1 on addition of plasmid DNA (b) Quenching of SYTO9 fluorescence in the presence of varying concentrations of MMGP1.

    Article Snippet: The plasmid DNA Bluescript II SK (+) was purified using a QIAprep Spin Minprep kit (Qiagen, Germay) and used for subsequent analysis.

    Techniques: Fluorescence, Plasmid Preparation

    (a) Treatment of DNA–MMGP1 complexes with proteinase K resulted in detection of plasmid DNA band on the agarose gel. C-Control SK+ plasmid DNA; 1-DNA–MMGP1 complex formed at 0.576 µM concentration of peptide; 2-DNA–MMGP1 complex treated with proteinase K (b) DNase I protection assay. The plasmid DNA (100 ng) was preincubated with the varying concentrations peptides such as, C+-no peptide; 1-0.576 µM; 2-0.288 µM; 3- 0.144 μM; 4-0.072 µM; 5-0.036 µM, respectively followed by treatment with DNase I. The DNase treated plasmid DNA was used as negative control (C-).

    Journal: PLoS ONE

    Article Title: Mechanisms of the Antifungal Action of Marine Metagenome-Derived Peptide, MMGP1, against Candida albicans

    doi: 10.1371/journal.pone.0069316

    Figure Lengend Snippet: (a) Treatment of DNA–MMGP1 complexes with proteinase K resulted in detection of plasmid DNA band on the agarose gel. C-Control SK+ plasmid DNA; 1-DNA–MMGP1 complex formed at 0.576 µM concentration of peptide; 2-DNA–MMGP1 complex treated with proteinase K (b) DNase I protection assay. The plasmid DNA (100 ng) was preincubated with the varying concentrations peptides such as, C+-no peptide; 1-0.576 µM; 2-0.288 µM; 3- 0.144 μM; 4-0.072 µM; 5-0.036 µM, respectively followed by treatment with DNase I. The DNase treated plasmid DNA was used as negative control (C-).

    Article Snippet: The plasmid DNA Bluescript II SK (+) was purified using a QIAprep Spin Minprep kit (Qiagen, Germay) and used for subsequent analysis.

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Control, Concentration Assay, Negative Control